genetic transformation

The text introduced the application of transgenic technology in the national and abroad in brief, meanwhile it summarized the genetic transformation mediated by Pollen-tube pathway, agrobacterium tumefaciens and particle bombardment.

简要介绍了转基因技术在国内外的应用，同时概述了大豆遗传转化主要采用的花粉管通道法、农杆菌介导法和基因枪法。

Technical improvements in somatic cell hybridization, such as genetic transformation, provide a promising approach for breeding new tolerant cultivars of this species, good protoplast culture system is fundamental and restriction point.

植物体细胞杂交技术的产生和发展为草地早熟禾新品种选育提供了一条新途径，良好的原生质体培养体系是利用体细胞杂交技术进行品种改良的根本和制约点。

The system of particle bombardment for wheat genetic transformation has been established and was most widely used.

基因枪法是小麦转基因最常用的方法，其转基因体系已基本建立。

We introduced the researches and some advances of medicinal plant biotechnology on tissue culture. protoplast culture. fusion and genetic transformation.

本文从组织培养、原生质体培养及其融合和遗传转化三个方面介绍了药用植物生物工程学的研究内容及其某些进展。

Such embryogenic cell suspension culture will provide ideal donors for induced somatic mutation, somatic hybridization, and genetic transformation.

这样的胚性细胞悬浮培养系将成为细胞水平突变、体细胞杂交和遗传转化的理想供体。

Genetic transformation in Bacillus pumilus ⅰ. selection of the competent strain

短小芽孢杆菌的遗传转化&Ⅰ.感受态菌株的筛选

High-efficient monocot promoter of Ubiquitin was used and results of enzyme digests and PCR analysis showed that the vector was well established and suitable for the genetic transformation of cereal crops.

选用单子叶植物高效表达启动子玉米Ubiquitin启动子，经酶切、PCR鉴定表明：该载体构建正确，适合于禾谷类作物的遗传转化。

Genetic Transformation of Brassica napus with in planta Method

用in planta方法转化甘蓝型油菜

Scientists have used Agrobacterium-mediated genetic transformation to generate transgenic plants, and this method depends on the Ti plasmid.

在过去的几十年间，研究人员用农杆菌转化法获得了多种转基因植物，该转化法依赖于农杆菌中存在的Ti质粒。

Gene gun bombardment has been widely used in wheat genetic transformation, for the virtues of no host-limit, wide target receptors, controllable and easy to operate.

基因枪法是小麦遗传转化中最为广泛使用的一种方法，它具有无宿主限制、靶受体类型广泛、可控度高、操作简便快捷等优点。

The precondition of Agrobacterium-mediated genetic transformation is to establish a high frequency regeneration system.

而建立高效再生体系是利用根癌农杆菌进行遗传转化的前提。

Genetically modified crops are obtained via the genetic transformation of crop genome.

转基因作物是应用生物技术对农作物基因组进行改造获得的。

The main problems and countermeasures of the present conditions particle bombardment, Agrobacterium mediated, pollen tube pathway of application in wheat genetic transformation are discussed.

讨论了基因枪法、农杆菌介导法和花粉管通道法在小麦转基因中的应用现状及其存在的主要问题与对策。

The expression vector was introduced into the receptor plant by genetic transformation method, and transformed offspring were identified, and the expression level of PEPC was analyzed from transformed progeny plants and transgenic plant protein, fat, fat composition changes were analyzed.

通过转基因技术将遗传表达载体转化进入受体植株，并对转化后代进行鉴定，并分析转化后代植株PEPC基因表达量的变化以及转基因后代植株的蛋白质、含油率、脂肪酸成分的变化。

The methods for wheat genetic transformation such as particle bombardment, pollen-tube pathway, ion-irradiation and Agrobacterium-mediated transformation were reviewed in this paper, and the prospects were discussed as well.

综述了基因枪法、花粉管通道法、离子束介导法、农杆菌介导法在小麦遗传转化上的应用及发展，展望各种转化技术的应用前景。

Application of a method for the genetic transformation in monocotyledon via the pollen tube pathway

花粉管通道法在单子叶作物遗传转化上的应用

Genetic transformation with the in planta method has greatly promoted researches of functional genomics in model plant, Arabidopsis.

用in planta方法进行遗传转化极大地促进了模式植物拟南芥的功能基因组研究，但在农作物中仍少有in planta的成功报道。

Advances in Agrobacterium tumefaciens-mediated genetic transformation of forest trees

利用根癌农杆菌转化木本植物的研究进展

Genetic Transformation of Cotton Embryogenic Callus by Particle Bombardment and Regeneration of Transgenic Plants

基因枪法转化棉花胚性愈伤组织获得转基因植株

The gene engineering of aspergilli has become increasingly active, and their genetic transformation systems turned to be essential.

对丝状真菌曲霉的基因工程研究日益活跃，而其遗传转化系统是进行基因工程研究必不可少的手段。

The Genetic Transformation and Genetically Engineered Cross Protection of CaMV Gene ⅵ in Arabidopsis Thaliana

CaMV基因Ⅵ在拟南芥上的遗传转化及交叉保护

Research Progress of Ornamental Orchid Plants in Tissue Culture and Genetic Transformation

观赏兰科植物组培快繁及遗传转化的研究进展

Studies on Establishment of Regeneration System, Expression of LEAFY Gene and Genetic Transformation in Ginkgo Biloba

银杏再生体系建立和LEAFY基因表达、转化的研究

Genetic Transformation of Soybeans by Soaking Its Seeds with the Total DNA of American Ginseng ( Panax quinquefolium) ⅱ. Determination of Isoflavone Content by HPLC

西洋参DNA导入大豆实现遗传转化的研究Ⅱ.导入后代异黄酮类成分的含量测定

Wheat Genetic Transformation Via the Pollen-tube Pathway and the Analysis of Gliadin in Mutant; an insect that carries pollen from one flower to another.

花粉管通道法遗传转化小麦与变异株的醇溶蛋白分析从一朵花到另一朵花传授花粉的昆虫。

A genetic transformation study of introducing wild Gastrodia elata DNA into Solanum tuberosum by soaking seedling method

利用浸苗法将野生天麻总DNA导入马铃薯的研究

We established genetic transformation system of lily callus ( Lilium 'Star Gazer') and leaves ( Lilium' Siberia') mediated by Agrobacterium tumefaciens.

通过根癌农杆菌介导的遗传转化方法，建立了凝望星空百合（Lilium‘Star Gazer’）愈伤组织和西伯利亚百合（Lilium‘Siberia’）叶片的遗传转化体系。

Research on Genetic Transformation of Bacillus Brevis

短芽孢杆菌的转化方法

Application of a method for the genetic transformation in plant via the pollen-tube pathway

花粉管通道法在植物遗传转化中的应用

At present, transgenic technique applied to cotton genetic transformation includes particle bombardment, Agrobacterium-mediated transformation and pollen tube passageway among many transgenic techniques, the former two technique need to establish genetic transformation system.

目前转基因的方法很多，在棉花的遗传转化中应用的主要有基因枪法、农杆菌介导法和花粉管通道法，其中前两种方法都需要建立基因转移的受体系统。